Phosphorylation Generates Different Forms of Rotavirus NSP5
نویسندگان
چکیده
منابع مشابه
Rotavirus NSP5 phosphorylation is up-regulated by interaction with NSP2.
We have previously shown that a number of isoforms of the non-structural rotavirus protein NSP5 are found in virus-infected cells. These isoforms differ in their level of phosphorylation which, at least in part, appears to occur through autophosphorylation. NSP5 co-localizes with another non-structural protein, NSP2, in the viroplasms of infected cells where virus replication takes place. We no...
متن کاملIn vivo and in vitro phosphorylation of rotavirus NSP5 correlates with its localization in viroplasms.
NSP5 (NS26), the product of rotavirus gene 11, is a phosphoprotein whose role in the virus replication cycle is unknown. To gain further insight into its function, we obtained monoclonal antibodies against the baculovirus-expressed protein. By immunoprecipitation and immunoblotting experiments, we showed that (i) NSP5 appears in many different phosphorylated forms in rotavirus-infected cells; (...
متن کاملAssociation of rotavirus viroplasms with microtubules through NSP2 and NSP5.
Rotavirus replication and virus assembly take place in electrodense spherical structures known as viroplasms whose main components are the viral proteins NSP2 and NSP5. The viroplasms are produced since early times after infection and seem to grow by stepwise addition of viral proteins and by fusion, however, the mechanism of viropIasms formation is unknown. In this study we found that the viro...
متن کاملRotavirus nonstructural protein NSP5 interacts with major core protein VP2.
Rotavirus is a nonenveloped virus with a three-layered capsid. The inner layer, made of VP2, encloses the genomic RNA and two minor proteins, VP1 and VP3, with which it forms the viral core. Core assembly is coupled with RNA viral replication and takes place in definite cellular structures termed viroplasms. Replication and encapsidation mechanisms are still not fully understood, and little inf...
متن کاملProkaryotic Expression of NSP5 Gene of Human Group B Rotavirus and Generation of its Polyclonal Antibody
A pair of primers were designed according to the nsp5 gene of human group B rotavirus strain WH-1, and the nsp5 gene was amplified by PCR and subcloned into the expression vector pGEX-KG. After induced with IPTG, the NSP5 was expressed as a GST fusion protein in the soluble form. Then the protein was purified and immunized mice to obtain specific antiserum. At last, polyclonal antibody was anal...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1996
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-77-9-2059